cell signaling technology 9139 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc cell signaling technology 9139
Cell Signaling Technology 9139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 3319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+signaling+technology+9139/Stat3+Mouse+mAb/pm41882003-400-46-46
Average 97 stars, based on 3319 article reviews

cell signaling technology 9139 - by Bioz Stars, 2026-09

97/100 stars

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Immunohistochemistry:

Article Title: Blockade of deubiquitinating enzyme PSMD14 overcomes chemoresistance in head and neck squamous cell carcinoma by antagonizing E2F1/Akt/SOX2-mediated stemness.
Article Snippet: The following antibodies are used for immunoblotting (IB), immunoprecipitation (IP), immunohistochemistry (IHC), immunofluorescence (IF) and Chromatin immunoprecipitation (ChIP) assay in this study: PSMD14, Proteintech (Rosemont, IL, USA), 12059-1-AP (IB, 1:1000); PSMD14, Sigma-Aldrich (St. Louis, MO, USA), HPA002114 (IHC, 1:400); PSMD14, Santa Cruz (Dallas, Texas, USA), sc-100464 (IF, 1:200); PARP, Cell Signaling Technology (Danvers, MA, USA), #9542 (IB, 1:1000); Caspase-3, Abcam (Cambridge, UK), ab32351 (IB, 1:1000); Cleaved Caspase-3, Cell Signaling Technology, #9661 (IHC, 1:400); SOX2, Proteintech, Theranostics 2021, Vol. .. 11, Issue 6 http://www.thno.org 2657 11064-1-AP (IB, 1:1000; IF, 1:200; IHC, 1:200); Akt, Cell Signaling Technology, #4685 (IB, 1:1000); Phospho-Akt (Ser473), Cell Signaling Technology, #4060 (IB, 1:1000); E2F1, Abcam, ab179445 (IB, 1:1000; IP, 1:100; IHC, 1:200; ChIP, 1:500); Ubiquitin, Cell Signaling Technology, #3936 (IB, 1:1000); Ki67, Cell Signaling Technology, #9449 (IHC, 1:500); Erk1/2, Cell Signaling Technology, #4695 (IB, 1:1000); Phospho-Erk1/2 (Thr202/Tyr204), Cell Signaling Technology, #4370 (IB, 1:1000); Stat3, Cell Signaling Technology, #9139 (IB, 1:1000); Phospho-Stat3 (Tyr705), Cell Signaling Technology, #9145 (IB, 1:1000); GAPDH, Santa Cruz, sc-365062 (IB, 1:5000) and Normal Rabbit IgG, Cell Signaling Technology, #2729 (IP and ChIP). .. 4-Nitroquinoline 1-oxide (4-NQO) was obtained from Sigma-Aldrich, MG132, CHX and MK2206 were purchased from Selleck (Shanghai, China), and Thiolutin (THL) was purchased from Tocris Bioscience (Avonmouth, Bristol, UK).

Chromatin Immunoprecipitation:

Ubiquitin Proteomics:

other:

Article Title: Hypoxia-induced miR-5100 promotes exosome-mediated activation of cancer-associated fibroblasts and metastasis of head and neck squamous cell carcinoma.
Article Snippet: The following antibodies are used for immunohistochemistry (IHC), immunoblotting (IB), immunofluorescence (IF) and Chromatin immunoprecipitation (ChIP) assay in this study: HIF1α, Abcam (Cambridge, UK), ab51608 (IB, 1:1000; IHC, 1:400); α-SMA, Abcam, ab7817 (IB, 1:1000; IHC, 1:400; IF, 1:200); E-Cadherin, Proteintech (Rosemont, IL, USA), 60335-1-1 g (IB, 1:1000; IHC, 1:150); N-Cadherin, Proteintech, 22018-1-AP (IB, 1:1000; IHC, 1:200); Vimentin, Cell Signaling Technology (Danvers, MA, USA), #5641 (IB, 1:1000); Zeb1, Proteintech, 21544-1-AP (IB, 1:1000); Snail, Cell Signaling Technology, #3879 (IB, 1:1000); Slug, Cell Signaling Technology, #9585 (IB, 1:1000); Twist, Abcam, ab50581 (IB, 1:1000); QKI, Proteintech, 13169-1-AP (IB,1:1000; IHC, 1:400); ALIX, Santa Cruz (Dallas, Texas, USA), sc-53540 (IB, 1:1000); CD63, Santa Cruz, sc-5275 (IB, 1:1000); CD9, Santa Cruz, sc-13118 (IB, 1:500); AKT, Cell Signaling Technology, #4685 (IB, 1:1000); Phospho-AKT (Ser473), Cell Signaling Technology, #4060 (IB, 1:1000; IHC, 1:300); STAT3, Cell Signaling Technology, #9139 (IB, 1:1000); Phospho-STAT3 (Tyr705), Cell Signaling Technology, #9145 (IB, 1:1000; IHC, 1:300); Cortactin, Abcam, ab33333 (IF, 1:400); GAPDH, Santa Cruz, sc-365062 (IB, 1:5000) and Normal Rabbit IgG, Cell Signaling Technology, #2729 (ChIP).

Article Title: Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction.
Article Snippet: Antibodies were conjugated to protein G beads (LYN) or protein A beads (TYK2) (Invitrogen), crosslinked using disuccinimidyl suberate (Pierce) as per manufacturer’s protocol, incubated with lysates overnight at 4°C, washed eight times with IP lysis buffer supplemented with 200 mM NaCl, and eluted by incubation in LDS sample buffer with 50 mM DTT at 70°C for 10 minutes.

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